Spin Trapping Technique Studies on Active Oxygen Radicals from Human Polymorphonuclear Leukocytes during Fluoride-stimulated Respiratory Burst

By means of the ESR spectrum technique it was demonstrated that fluoride (F-) stimulated human polymorphonuclear leukocytes (PMN) in a respiratory burst to generate active oxygen radicals ( • 02 and -OH) and to consume oxygen (02). The experiments' using the spin trapping technique with the nitrone DMPO as the trapping agent indicated that the PMN produce the adduct DMPO-OOH under high concentrations of Fand the adduct DMPO-OH under low concentrations of F-. Under medium concentrations of F-, DMPO-OOH was produced which then decreased in amount as a decrease occurred in Fconcentration. The results of tests of scavenging by superoxide dismutase (SOD) and catalase suggested that the active oxygen radicals generated in the respiratory burst originated mainly from superoxide (-02). By the spin probe oximetry method (CTPO and CrOX) it was shown that the 02 consumed is from the intercellular medium.